What’s Inside?
The gel in a FermProbe^® is a non-toxic, USP grade, organic material. Other manufacturers may use different materials such as polyacrylamide. Please call us for more information or an MSDS.

Why Is The Gel In The Probe Turning Brown?
Over time, the high temperatures of sterilization will discolor the gel in a FermProbe. This is often referred to as carmelization. It is normal, and in no way affects the performance of the electrode. This color change can also be used to approximate the age of the electrode.

What Is The Shelf Life?
FermProbes can be stored up to one year from date of shipment, with no appreciable change in their performance or expected service life. Check the date code stamped on the connector or integral cable, and rotate inventory regularly.

How Old Is This Probe?
All FermProbes have a three-digit date code stamped on the connector or integral cable. This indicates when the electrode was produced. The first digit is the year, and the last two are the month. For example, a FermProbe with a “007” date code was manufactured in July of 2000.

What Are The Crystals On The Probe?
During storage, salt crystals will often form on the electrode, around the soaker bottle. Sometimes the formation is so extensive there will be crystals under the soaker bottle and on the foam inside the box. This will not affect the performance, accuracy or life of the probe. Rinse off the crystals with water prior to use.

How Should FermProbes Be Stored?
Never store a pH electrode dry. The first choice for storing FermProbes is KCl solution. We recommend any concentration between 2.0 M and 3.8 M. Another good choice is buffer solution. A pH 4 buffer solution is best since it will keep longer, but pH 7 is also acceptable. If it is necessary to clean a FermProbe with acid, caustic, solvent or other cleaning solution, it is best to soak the electrode in KCl solution after cleaning and prior to use or calibration. This will re-condition the bulb and reference, extending the probe life and improving calibration accuracy.

How Should A pH Electrode Be Installed?
All pH electrodes (and DO sensors) can be mounted vertically, into a vessel headplate. Electrodes and sensors can also be installed on large vessels, through a side port. For side-entry installations, the probe must be at least 15° above horizontal to insure proper operation. Remember, there is liquid inside all pH electrodes and DO sensors. This slight 15° incline is enough to prevent a bubble from lodging itself at the tip of the sensor, preventing the sensor from operating properly.

What Are The Three Most Important Things To Remember During Calibration?
1) Always use fresh buffer. The pH of buffers will change quickly due to air, dilution and carry-over.
2) After rinsing a pH electrode between buffers, blot the electrode dry, do not wipe it. Rubbing the electrode can build up a static charge, just like rubbing your feet on the carpet. This charge can prevent proper calibration. (If this happens, wait and let the electrode stand in solution so the charge can dissipate.)
3) Always consider the effects of temperature on the measurements. Many pH measurements are temperature dependent. Please call us for a copy of our newsletter on temperature compensation and pH measurements.

How do I acheive more accurate pH measurement?
To yield the most accurate pH measurement, it is important to compensate for temperature. Temperature has two significant effects on pH readings. The solution pH and the electrode output will both change at different temperatures. These two effects, either together or separately, can lead to errors in calibration, measurement and control.

How should I install a pH Sensor?
A pH sensor must be at least 15° above horizontal to consistently function properly. DO NOT install sensors in a port perpendicular to the vessel wall. The liquid in the sensor contains small air bubbles. If not inclined slightly above horizontal, a bubble can adhere to the pH bulb where it will affect the sensor’s performance.

How is the housing length determined?
The typical insertion length is calculated from the top of the threads to the furthest point, either the guard or the sensor tip.

How should I clean my FermProbes?

FermProbes which are physically intact can often be restored to an improved level of performance by one of the following cleaning procedures:

1. General: It is good practice to wash the tip of the electrode with a solution made from dish soap and/or enzyme detergent and warm clean water. An alternative is to use a caustic, such as Sodium Hydroxide, or CIP 100, typically a diluted solution with a pH of around 12 to 13 pH. Use a soft toothbrush or a clean soft cloth wetted in the solution to carefully scrub the tip of the electrode. Thoroughly rinse the electrode tip with distilled or D.I. water and soak in KCl solution, 2M or stronger, for a minimum of 30 minutes before recalibration or returning electrode to service. Do not store in distilled or D.I. water.

2. Inorganic Deposits: Try to dissolve deposit by immersing the electrode bulb in 0.1N Hydrochloric acid for a few minutes followed by a thorough rinse with D.I. or clean tap water. Then proceed with the general cleaning in Step 1 of this section.

3. Organic Oil, Grease Films or Fingerprints: Wash electrode bulb with the solution in Step 1 of this section. Wash the tip with acetone. Follow with the general cleaning procedure in Step 1 of this section. Note: Depending on the extent of the oil and/or grease contamination, the electrode may be damaged beyond recovery.

4. Plugged or Dry Ceramic Liquid Junction: Try at least one of the previous 3 steps in this section. Place the electrode in KCI solution, 2M or stronger. Heat slowly to 50ºC then let cool to room temperature. Repeat as necessary.